My peak intensity table data can't pass data integrity check with statistical analysis (one factor)

MetaboAnalyst
1

Hi everyone,

I am a PhD student at UNSW currently working with LC–MS metabolomics data. I have been struggling to process my peak intensity table in MetaboAnalyst, as my dataset consistently fails the integrity check.

My dataset includes:

  • 5 pooled quality control (QC) samples

  • P. aeruginosa treated under the following conditions:

    • Drug A (1 hr, 3 hr)

    • Drug B (1 hr, 3 hr)

    • Combination of Drug A and Drug B (1 hr, 3 hr)

  • Each condition contains six biological replicates

I would greatly appreciate any guidance on how to properly format and structure my data table so that it passes the integrity check in MetaboAnalyst. Additionally, I would be very grateful for recommendations on appropriate data filtering and normalization settings for this type of dataset.

Thank you very much for your help!

2

That is really frustrating! Dear Omics forum teams, could you help me please?

3

Please read and follow the post guide