MEBA not working - data unbalanced

MetaboAnalyst
1

Hello,

I am working on statistical analysis [metadata table] module. In particular, I use time-series + one factor design. My data are expressed as concentrations.
I have 3 different treatments (Phenotypes), 4 time-points (Time), and 4 Subjects per phenotype-time point. Therefore 48 samples in total. These are the .csv files I use:
Data_MA_meba issue.csv (9.0 KB)
Meta_MA_meba issue.csv (871 Bytes)

I do not normalize the samples or transform the data, but I apply auto scaling.
The data check detected 0 missing values, and all the statistical analyses are working (ANOVA2, ASCA, Heatmap, etc).
When I try the MEBA, I get this error:

  • Error Please make sure data are balanced for time-series analysis. In particular, for each time point, all experiments must exist and cannot be missing!

The point is that the data are balanced and no experiment is missing, I checked the matrix over and over again.

Has anyone experienced the same problem? Any suggestions as to what the error might be?

Thanks in advance for your help.

2

Please follow our post guidelines

3

Hello Jeff, I updated the topic following the guidelines

4

The issue is fixed and you can try again after server update during the weekend. It is related to weak signals in your data (i.e. nothing pass the default threshold).

5

This topic was automatically closed after 3 days. New replies are no longer allowed.