A similar question on pathway direction for targeted metabolomics can be found here. Overall, there is no reliable metrics nor biologically meaningful interpretation on the overall pathway direction (please provide references you know on this topic).
In my view, the most useful approach is always thinking about your data, the question, and visualization (with some assistance from statistics). MetaboAnalyst allows you to visualize every LC-MS peaks invovled in a pathway, it is up to you to provide interpretation (and validation). See Figure 5 from our 2022 Nature Protocol
