Hello,
I’m looking for a metabolic pathway analysis based on VIPs from a PLS model (continuous variable). After transforming and normalizing the data, the “Select a pathway library” and “Specify pathway analysis parameters:” menus appear. As the samples are mouse blood, I’d like to use the mus musculus library (KEGG) and make a scatter plot (global test, relative betweeness centrality). However, I get an error message. Surprisingly, when I select the homo sapiens library (KEGG) for the same data set, the analysis works. Similarly, I can produce a heatmap by selecting the mus musculus library. This seems to indicate that my dataset is well supported by the application. Could someone please explain why pathway analysis doesn’t work when I select the mus musculus library (KEGG) while my dataset works for heat maps or when I use the homo sapiens library for pathway analysis?
Thank you very much for your help.
Yann
Yann