I’ve been working on functional meta-analysis with peak intensity table but have encountered an issue while uploading my data. My dataset, saved as a CSV file, includes m/z and RT values structured similarly to the example data provided by the software. However, when I upload it, the process fails and returns the following error message:
Possible causes of error (last one being the most relevant): Make sure that feature names are numeric values (mass or m/z)!”
I ensured that the feature names in my data were formatted as numeric values for m/z and RT using the formula “m/z_RT,” following the example data format. Despite this, the software still does not accept it.
To troubleshoot, I tried the example data in two ways:
Clicking to select “malaria” or “COVID-19” data directly and submitting: it worked successfully.
Downloading the “malaria” or “COVID-19” data to my computer and re-uploading it: this resulted in the same error message as with my own data.
This suggests the problem occurs even with example data when downloaded and re-submitted.
I’m wondering if this might be a software bug or an issue specific to my computer setup. Has anyone else encountered this issue? If so, do you have any suggestions for resolving it?
I’ve uploaded a shortened version of my data table here to help you more easily identify the potential issue.
This is because your data contains only 4 peaks. Mummuchog expects the complete peaks list from high-resolution LC-MS (usually >1000s peaks). You can see more discussions in this post
I also tested our example data, it works as expected. As there could be many ways that cause an error despite the best intention, please follow our post guideline if you believe the issue is related to our example data or tools (not your data or operations)
Thank you, Dr. Xia. The uploaded data is just an example to show you the structure of my data table. My actual data table contains around 2,000 peaks. However, I realized I made a silly mistake—I included the retention time (RT) in my data but left the “Retention Time” field marked as “Not Present.” That’s why it didn’t work initially.
Now, I’ve corrected the issue, and the data was successfully submitted. However, I encountered another problem: the results show “no records found.” Do you have any suggestions for resolving this?
I’ve also attached a shortened version of the data table here to help identify the issue more easily. Data for analyze-sample-2.csv (136.1 KB)
I’ve encountered another issue with my data, which includes results for “positive,” “negative,” and “HILIC” ions.
I was able to successfully perform functional analysis on my peak list (m/z, p-value, t-score, and RT) for positive ions. However, I would like to analyze all the peak lists (positive, negative, and HILIC ions) together. To do this, I created a combined peak list and selected the following settings: Ion mode as “Mix mode,” Mass tolerance as “5 ppm,” and Retention time as “Yes-minute.” Unfortunately, the platform returned an “unknown error.”
I’m wondering if it’s possible to analyze all the ions together as described, or if I need to analyze them separately as individual peak lists for “positive,” “negative,” and “HILIC” ions. If separate analysis is required, which ion mode should I select for the HILIC ion peak list?
I’ve attached my combined peak list data for all three ions and would greatly appreciate your guidance.