Failed to download volcano image

[Luigi]

Uploaded data on MetaboAnalyst 6.0 browser for paired statistical analysis (one factor, samples in colums, paired, peak intensities) of lipidomic results on a Mac (Sonoma 14.5). No issues with upload or any output but the Volcano. The report is there, I can download the csv file from the download section, and I can see the actual interactive vulcano (see image). But i cannot download the figure (see images). I have deleted cookies and cache from the browsing story.

Can you please advise? Thanks

[jeff.xia]

Please follow our post guideline

[Luigi]

1. Which tool and which module

• incomplete: only tool name provided
  https://www.metaboanalyst.ca/Secure/analysis/VolcanoView

2. Provide a copy of your data (the actual data file, not a screenshot image of the data), or indicate which example data you used
   Uploaded

3. Document all steps leading to the issue. Sometimes screenshots may be necessary
   - Metaboanalyst/Statistical analysis (one factor)
   - Peak intensities/samples in columns (paired)/choole file/submit
   - Proceed
   - Proceed
   - Sample normalization: none/ Data transformation: Log transformation (base 10)/ Data scaling: Pareto scaling/ Normalize/ Proceed
   - Volcano
   - Paired/ minimal/ all significant/ FC 1.5/ Direction 1/0 / pVal 0.1 Raw/ Submit
   - Customize the data output/ PNG 300dpi 12 inches/ submit
   - Download the image/ HTTP Status 404 - Not Found

4. If it is about using R packages, you also need to provide the environment information, such as
   N/A

5. If it is about raw data processing, please also describe how the data were collected (instrumentation, etc).
   N/A

If your questions are not answered, it is probably because we don’t know how to reproduce the issue, or we don’t have time to guess all possible scenarios.
I hope the issue now clear. The problem is only with downloading the actual plots and table of result, not with seeing them on the screen. Thanks.
MetaboUPLOAD.csv (1.8 KB)

[jeff.xia]

Thank you for the details. I did not notice any issues based on the steps. It generated the graphics as expected for downloading. However, the volcano plot is different from your screenshot (see below).

[Luigi]

Thanks!

My mistake, I gave you the wrong spreadsheet - that one it’s actually working.
MetaboUPLOAD2.csv (1.5 KB)

Please, find on attachment the one I cannot make working - the same with one sample pair less. Could you please help?
Thanks.
-Luigi

[jeff.xia]

I can see the issue is related to one of your features (see below) - which has identical values in each group, violating t-test model assumption (i.e. normal)

You can

1. Remove this feature and perform analsis as before
2. Keep this feature and perform analysis using non-parametric method (i.e. use the checkbox in the Volcano plot)
3. Wait for the next update (in 2~3 days) - which will first perform regular t-tests. For those failed t-tests, perform non-parametric tests (i.e. Wilcoxon signed-rank test)