ANOVA2 Generation Issues Continued

Hello there,

I made a post about this in February (24 days ago). However, I’m seeing continued issues with this feature of MetaboAnalyst so I wanted to post again asking for help.

I’m using the online MetaboAnalyst software, specifically using the statistical analysis [metadata table] tool. I am trying to do a time-series + 1 factor analysis, and I’ve been uploading the data sheet and metadata sheet attached below.

Here are the steps I’ve been taking:

  1. statistical analysis [metadata table]
  2. data type: peak intensities, study design: time-series + one-factor, data format: samples in rows, upload the data sheet and metadata sheet attached below
  3. Data filtering: none
  4. data scaling: auto scaling
  5. Multi-factor ANOVA
  6. Select two metadata: phenotype, type, Adjusted p-value cutoff: 0.05, phenotype factor: within subjects… Submit.

Each time, I get the error shown in the screenshot below, “unknown error occured”. For some reason, ANOVA2 is the only tool that doesn’t work: I’ve done iPCA, Heatmap2, Linear Model, Correlations, and RandomForest all with success.

On another data set, I’m running into the same issue when I try to run a simple time-series only ANOVA2.

In my previous post, jess.ewald had fixed the issue temporarily, explaining, “there was an update to an R package that changed the format of the results table (thus breaking our interface)”. I wonder if the same thing is occuring now?

Thanks so much for your help!


Data Sheet for MA Help Forum, Time Series + 1 Factor.csv (161.0 KB)

Metadata Sheet for MA Help Forum, Time Series + 1 Factor.csv (2.7 KB)