Hello,
I’m observing an error when performing One-way ANOVA analysis in the Statistical Analysis [one factor] module. Specifically if I upload data in which the samples are not sorted by group (see attached: P24_0802_Exp_1_combined_for_MA-group_1_to_4_only.csv) I observe no statistically significant features.
If I sort the data by group (see attached: P24_0802_Exp_1_combined_for_MA-group_1_to_4_only_group_sorted.csv) then I get a very different result with around 1000 significant features at 1% FDR. This is also the result that I get on my MetaboAnalyst Pro account and is more in line with what I would have anticipated from this dataset.
I suspect that the samples are being assigned to the wrong groups for the ANOVA analysis.
I’m having trouble providing the files here but if you would like them then please shoot me an email and I’ll send them through.
Kind regards,
Chris