MetaboAnalyst 6.0 Pathway analysis

[Kam]

I’m doing Pathway analysis using MetaboAnalyst 6.0, but I’m getting errors. Screenshots attached.

1. I uploaded the file in txt format.
   
   
2. After normalization, I chose the parameters for pathway analysis and selected Arabidopsis as KEGG Library for the analysis.
   
   
   
   
   
3. There is an error after proceeding.
   
   

[jeff.xia]

Please follow our post guideline

[Kam]

I have attached the actual file that led to the error.
S1.txt (2.4 KB)

[jeff.xia]

I checked your data (see below) - It contains 3 samples in one single group (S1), and you specify “continuous group labels” - note this is not valid categorical as it requires at least two groups. Please make sure you read our tutorials first. You can start from example data formats MetaboAnalyst