Why should I filter baseline noises (untargeted metabolomics)?

  • For NMR or MS spectra, there are many regions where no known compounds in biofluids have signals. The corresponding regions contain mainly baseline noises.
  • When signal approaches background, the relative errors increases and conclusions based on these data will be questionable.
  • The penalty for multiple testing adjustment will also become more stringent to control false positives.

Therefore, it is best to first try to filter baseline noises before further analysis.