Unknown error that is unique to your data and parameters but table and metadata seems correct

Please follow our post guideline. Saying it works a few months ago is not helpful.

@ hynr11: see below - you metadata contains two columns and neither is compatible with our data input format Sample column contain only one group, and Replicate should have at least 3 replicate per group. Please take a look at our data format requirements
Screenshot 2024-07-25 at 3.34.12 PM