I keep running into the “error unique to your data” troubleshooting error page.
This is in the Metaboanalyst-> Functional analysis[LC-MS] page.
I have my mass and retention time in the mass__ret format like the malaria example in the formatting guide.
Have all unique samples, no copy names, and each has its group marked as the malaria example.
To get to the error, you select peak table, retention time available in seconds, samples in columns and upload the data file (.csv). The other options kept as default.
It passes the data integrity checks, the variance and abundance filter options dont seem to effect the error outcome and may be skipped to find the error.
Data normalized by log base 10 transformation and auto scaling.
Here at the parameter section, I get the warning " Warning- Total of 3 groups found - ANOVA will be used for selecting significant peaks! Using ‘Data Editor’ to include 2 groups is recommended for easy interpretation" pop up and select the G.max option in plants and upon proceeding I get the error.
Im not sure of a better way to process the data that may mitigate this issue or a better tool in this system to run the data. Thank you all for any help. Ive included my data file to help.
testmetabolomic.csv (341.9 KB)