T-test analysis error

I am facing error in Statistics-one-facto
I am uploading data matrix in desired format and after that missing value estimation, then Normalization of data to gaussian distribution of data, then going for T-test analysis it is showing error.
while FC (fold change) is working.

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Unless you show the issue using our example data (which one), you must provide your data so we can reproduce the issue - which could be due to unique issues in your data