Hello!Can I use the Volcano plot results and the VIP score from the PLS-DA model to select significant metabolites separately? Or do they always need to be used together? Suppose if metabolite A has p<0.05, FC>1.5 and metabolite B has VIP>1.5, do I have the right to say that both of these metabolites are differential metabolites? Thank you.
All these metrics (FC, t-tests or VIP) measure different things with different underlying assumptions (and limitations). They could be individually sufficient when you use them in the right context.
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