Sample normalization or co-variate?

My metabolomics analyses were run on subsamples of homogenized whole fish. Anyone know if I should normalize individual samples by fish weight or if fish weight should be considered a co-variate/entered as meta data?

If you expect fish weight could affect metabolomics profile, you can include it as a covariate. I assume you inject the same volume/mass for metabolomics (disregarding the fish weight)

1 Like