Reads my paired-end library as single-read library! Why?

If I click No, the popup disappears. If I click yes, it doesn’t go anywhere. Stuck at the first step after uploading files. How can I fix it? Thanks for the help.

Hello, could you please check if your unzipped files end with R1.fastq/R2.fastq and the metadata contains all the uploaded files?
If it is, please share with us several example files for further trouble shooting.
Hope this helps!

Here you can see the unzipped file names ends with R1.fastq and R2.fastq

I didn’t understand what do you mean by several example files?

I mean you can share with us the files you used for reproducing the problem. If they are large, you can share part of files as example.

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