Hi,
I have been using Metaboanalyst for a long time, but this is the first time I used the QC filtering option, since this is a fairly new option. I have been doing QC filtering manually on my excel and then uploading the data for normalization etc. This time I used the QC filtering option provided by the software and the data I got were vastly different from what I had obtained manually. I lost 40 metabolites (% RSD 25), while I lose 12 metabolites when I did manually. I am still trying to figure out what went wrong. Any tip helps.
Thank you,
Georgia