Dear all,
I’m performing Quantitative Enrichment Analysis (QEA) in MetaboAnalyst. I’m wondering about the utility of normalization, scaling, or data transformation at this specific step. Since QEA uses concentration or intensity values , does applying normalization or scaling (e.g., log transformation, pareto scaling, etc.) improve the biological interpretability or robustness of the enrichment results? I’ve noticed that in the literature, it’s not always clearly specified whether normalization or transformation should be applied before QEA, which adds to the uncertainty. I´d appreciate any insights. Thank you for your help! 