Pathway analisys for multi groups

Previously, we could perform pathway analysis with multi groups.
In this version of ours, is it not possible to perform with 3 groups?

The main reason is that the result will be hard to explain when there are multiple groups. The increase / decrease is best defined in two-group design. However, you can always first perform analyses that accept multiple groups (such as ANOVA) and then use the ranked peaks for pathway analysis.