Normalization issue (rarefaction)

Hello,
I am reaching out to seek clarification on an observation I’ve made regarding the normalization and rarefaction processes in our analysis. I’ve noticed the following:

  1. Once I apply rarefaction, no matter what normalization I chose, I always get the rarefaction number

Could you please help me understand why this occurs?
Thanks!

Please follow our post guide

Thank you and here are the files

microbiomeAnalyst_16s_meta.txt (1.4 KB)
microbiomeAnalyst_asv_seq.txt (3.5 MB)
microbiomeAnalyst_taxonomy_annotation.txt (3.1 MB)