Metabolite Pathway Analysis Comparison


I’m trying to perform pathway analysis on a metabolite dataset that I acquired and processed through NMR and assigned through chenomx, and I now have the dataset converted to csv. How does one perform pathway analysis for separate groups in order to compare the pathways of the different groups. As far as I understand, it only allows you to upload a dataset with two groups (doesn’t work with a single dataset, doesn’t work with more than two), but creates a sort of ‘all-in-one’ pathway analysis and no way of comparing the difference in pathways between the two groups.

Please excuse my ignorance around this as I am very new to analysing omics data. I essentially want to be able to compare pathways between conditions, but it won’t allow me to generate separate ones for individual conditions.

Many thanks in advance for any responses,