LC-MS database selection not working. It says peak construction failed at network construction step

The input file is in the same format as the example provided. (Mz Rt Int Pvalue)
The data is uploaded successfully.
When selecting KEGG database under peak-metabolite option, it says peak construction failed at network construction.
Please help.

Dear Grace,

I would need to use your dataset to reproduce the error.

Guangyan

Is there a way for me to send it to you privately? Thanks

Hi Guangyan.
I used a different dataset. Similar problem appeared. This time it says “peak processing failed at structure propagation step”. Please help.
Thanks,
Grace

Hey Grace,

The issue comes from the fact that you only uploaded a list of significant peaks but you need to upload the whole list (usually 10k), including those that have p-value more than 0.05.

Guangyan

Hi Guangyan,

I uploaded the full list and it still doesn’t work. Thanks.

Grace

There are many possible reasons that an error could happen. To start, please carefully read and follow our post guide

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