I am trying to re-input a data set I analyzed last month (one factor statistical analysis) with no issues (when metaboanalyst was still filtering to 5000 features, instead of 10,000 like it does now). I am using all the same settings and the same exact csv file (40% IQR filtering, Normalization by median, and auto-scaling) and now it will not even display the normalized data. If I continue on with the analysis, I can get to the fold change tab, but eveyrthing else throws an error. I have no idea why this might be but would be grateful for any help. I’ve attached the original file and screenshots of every step. Thanks!
4. 10% FCS vs 10% OASF.csv (483.7 KB)
Yes, this issue happens occasionally, especially when internet connection is slow. It is likely caused by a combination of large user traffic and internet congestion .
Please try again at different time with good connection.
@jeff.xia I don’t think that’s the issue, it only does it for specific files while others seem to work totally fine. Even if I plug them in right after the broken ones.
PS I hope you are actually seeing this reply. This is the third or fourth time I’ve had to redo my reply because it seems to dissapear from the post a day later every time I make one.
Everything works as expected. I can only suspect it is related to your local network environment. You may try different computer i.e., from work to home computer, or mobile device. You can also search the forum to see others comments/solutions on this matter
@jeff.xia It’s not my internet. I have tried all of those things, you name it and I have probably tried it. I had a friend who does similar work in a completely different state try it with the same files and she has the same issue as well. I have no problems with all but a few specific files. All of the other posts I am seeing that are similar are basically being told “there is no issue.” So you can see why I am at a completel loss here. And as soon as I set autoscale to “none” it works but the settings I used a month ago (normalize by median and auto-scaling) do not work anymore.
Even it works by default on my side, maybe you can try the most obvious (i.e. do not introduce space nor use special characters in naming / labeling in general)
- File name => FCS_OASF.csv
- Class names => FCS, OASF
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