How should I interpret a volcano-like plot from GSEA analysis (Functional Analysis module)

MetaboAnalyst
1

Hello all, I am using a GSEA approach to run pathway analysis on my metabolomics data. this analysis produces the plot that reminds me a volcano plot with pathways arranged according to their p-value on the y-axis and NES on the x-axis. On this plot NES (i.e. enrichment scores) can be negative or positive. How is this determined? How should I interpret this plot?

Best,
Lisa
ZA17_DH_GESA.pdf (111.2 KB)

2

Indeed, we use volcano plot as a visual summary of the GSEA result (based on its p values and NES). I guess your question is on how to interpret NES? I would refer you to the GSEA website. Here genes are replaced by putative metabolites

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