How should I build my OTU/ASV table (.txt, .csv or its zip) with the included sequences?

MicrobiomeAnalyst
1

I would like to try the newly added function (Tax4Fun 2.0) to predict functional profiles based on 16S rRNA data but I don’t know how to build the OTU/ASV table (.txt, .csv, or its zip) with the sequences included.
If someone could help me I would greatly appreciate it.

2

Hello,

Thanks for posing the question.

For using the tax4fun2, you need to include the sequences in the OTU/ASV table as the #NAME, e.g.

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The example can be found in our example dateset Pediatric IBD.

Make sure you select the check box Sequences included.

Screenshot 2022-12-01 at 17.13.50
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Hope this helps.
Yao

3

Hi,

My ASV data table is exactly looking like that, but I have received the following error:
“ErrorSequence information is needed for function prediction using tax4fun2”
I selected the check box “sequences included” in the beginning. Could you help me what would be the reason?
Thanks!

4

We have update the website. The issue is fixed now. You can try again.

Hope this helps!