Housekeeping gene tagged despite being above 0.05


I was running analyses on ecotoxxplorer, and noticed the gene H6PD would be tagged ** despite being 0.066. Is this normal / is it because it’s close to 0.05?

Thanks for letting us know, the cut-off for flagging housekeeping genes was recently changed from 0.1 to 0.05. We had changed the cut-off in the calculation part, but not the plotting function. I’ve updated and the change should be live soon.

Just to check - the p-value 0.066 was starred in the plot, but the “Housekeeping genes” quality check was not flagged, right?

Hello Jess,

Thank you for fixing the issue!

Yes, despite looking significant while plotted, it didn’t get flagged during the hkg check.

Hello Jess,

Happy New Year! I hope you’re well :slight_smile: I was analyzing some samples on EcoToxXplorer and one of the housekeeping genes was flagged again despite being > 0.05 (it was 0.08). I included a screenshot. The issue may not have been permanently resolved from last time.

Hi :slight_smile:

Can you provide a copy of your data and I will look into it? When I try the example data that has one HKG p-value of 0.083, it is correctly not being flagged:

You can also try clearing your browser cache and trying again - sometimes changes we make on our end are not available until you do this.