Housekeeping gene tagged despite being above 0.05


I was running analyses on ecotoxxplorer, and noticed the gene H6PD would be tagged ** despite being 0.066. Is this normal / is it because it’s close to 0.05?

Thanks for letting us know, the cut-off for flagging housekeeping genes was recently changed from 0.1 to 0.05. We had changed the cut-off in the calculation part, but not the plotting function. I’ve updated and the change should be live soon.

Just to check - the p-value 0.066 was starred in the plot, but the “Housekeeping genes” quality check was not flagged, right?

Hello Jess,

Thank you for fixing the issue!

Yes, despite looking significant while plotted, it didn’t get flagged during the hkg check.