Hi all,
peak_table_2.csv (15.1 KB)
I am trying to run the functional Analysis of metabolomics data generated from mouse livers, starting from the peak intensity table.
The table that I upload is a .csv file which contains the name of the features (MZ__RT) in the rows and the samples on the columns, as you can see from the file I uploaded here.
I normally skip the filtering (because I have less than 5000 features), and I remove the Quality controls and the blanks by editing the groups, before performing the data transformation (Log transformation). Finally I set the algorithm (I tried both Mummichog and GSEA), the parameters (p-value 0.1) and the pathway library (I tried both of the mouse libraries).
However when I try to proceed I get back the Generic error page for which I put a screenshot in the attached files.
I guess this error is due to an error in the data formatting, or because I only have 54 features in the table, and the algorithm cannot find any enriched pathway.
Can you please help me to solve this problem?
Thank you in advance.
Best,
Flavio