I am a Ph.D. student at UNR attempting to use MetaboAnalyst for functional analysis of my untargeted plant UHPLC-MS metabolic data. I’ve been struggling on uploading my feature lists for functional analysis. I have mixed ionization mode, and have been unable to successfully upload my simple feature list as a peak list. The peak list option is the only one that allows for ‘mixed ion mode’, specification. I have actually uploaded the same data as a peak table… but i am forced to select one ionization mode and that will result in skewed analysis. It is the same error over and over (see attached screenshot). I have messed around with formatting, following the same structure as the sample data but it is still not working. I have uplaoded only two columns , ‘mz’ and ‘p value’ ; ‘mz’ ‘rt’ ‘p value’ ; ‘mz’ ‘rt’ ‘ion’ ‘p value’ … Are there any suggestions or advise for uploading?
I figured out the issue. It was a formatting issue, where my column was labeled ‘ion’ instead of ‘mode’. I wish that the error wasn’t displayed on Unknown Error and actually displayed the proper error message. For example, if a mz column isn’t present or mispelled it will provide the error ‘Peak list requires a mz column’!’ .