Hello,
I am looking to combine transcriptomic data with metabolomic data obtained from drosophila melanogaster embryos. I have obtained a list of differentially expressed genes from the RNA-seq analysis, and I want to combine it with the DE results that I obtained from the Metaboanalyst statistical analysis module.
After selecting the Joint Pathway Analysis module from the online server, I would include both of the attached lists into their respective text boxes. I then selected entrez gene id and HMDB id for each of the id categories. After selecting Drosophila melanogaster as the model organism, I deleted all unmatched ids from the match results. All genes were found, but ~4 metabolites were not. In the Parameter Setting page, I am unable to produce results with integrated pathways. None of the algorithm settings seem to work always yielding an “Unknown Error Occurred” window. I have included both the DE gene and metabolite lists below. I look forward to your help.
metabolanalyst_de_metabolites_HMDB.txt (1.7 KB)
entrez_deg_list.txt (2.1 KB)