Data Filtering & Normalization - Inactive 'submit' button

FastBMD
1

The ‘submit’ button in ‘Data Filtering & Normalization’ seems to be inactive. Thanks!

2

Hi Marina,

The button is working when I run our example datasets through the pipeline. The problem is likely with your specific dataset. Would you be able to attach a copy of the data? Then I can find the specific problem and fix it.

Thanks!

3

Hello Jess,

Thank you very much for your help. Please, find the data attached.

Kind regards,
dosis_mirnas.txt (70.5 KB)

M.

4

Can you let me know exactly your data upload and selected normalization parameters (organism, data type, ID type, etc.)? Your data seems not standard microarray or RNA-seq data.

5

Hello Jess,

It is perhaps not the right type of data, I am not sure. These are the parameters I used:
Specify organism: H. Sapiens (human)

Data type: microarray data (intensities)

ID type: not specified

Gene-level summarization: mean

6

When your ID type is not supported, you should select ----Annotation-free Pipeline— option for both species and ID type.

Using these input options, I was able to analyze your dataset. I did not find any gene BMDs (none passed the quality filters). This can often happen when there are only three doses and not so many genes.

7

Hello! Thank you very much for the clarification, that’s really helpful.