Spectral analysis module of Metaboanalyst 6.0 seems to only accept files that are not larger than 50 MB. Each of my mzmL files is actually around 500-600 MB in size. Can you suggest any compression methods or any other alternatives for my spectral processing? Thanks.
Also, may I ask if other users are also experiencing difficulties signing in MetaboAnalyst? Thank you.
In spectral processing you can upload less than 200 MB size, but right now i am also facing some error after uploading mzML zip file and same for signing issue .
Thankyou
use metaboanalystR! metaboanalystR doesn’t have any limitation on file size and number.
I am having the same issue… my collaborator has tried using MetaboanalystR but it is still quitting halfway through. The only thing I have figured out is that using MSConvert to peak pick drastically reduces my data size, but I don’t think this is proper since the spectral processing already does peak-picking and I don’t think it should be done twice… if you find any answers please let me know!
I don’t know how many files you want to upload to MetaboAnalyst, but in my case I want to make a study of 145 files (there are 25 QC and 140 samples (duplicated)) with a mean of 30 MB each, which is a lot. MetaboAnalystR doesn’t work properly here because it would need a lot of memory, and then I tried to work it via cluster but I need to maximize the utility memory in order to the calculus to work.
Regarding the difficulties signing in MetaboAnalyst, I believe they are because it is server based. As the others have recommended, try to use MetaboAnalystR instead.