Alpha/Beta diversity modules failing on MicrobiomeAnalyst (Marker Data Profiling)

I am experiencing a consistent issue with the Alpha and Beta diversity modules in the Marker Data Profiling workflow.

My analysis includes 32 samples with different labels but belonging to a single group (one single column in the metadata). The OTU, metadata, and taxonomy files were generated using Microbat. If necessary, I can attach them.

Specifically:

  • Alpha diversity immediately redirects to the troubleshooting page without running the analysis

  • Beta diversity starts (“Processing…”) and then fails with a generic error

This happens:

  • even when using datasets that previously worked correctly

  • even when running without taxonomy

  • using minimal preprocessing (TSS normalization)

  • in a clean browser session (incognito, no extensions)

This suggests a possible server-side or backend issue rather than a formatting problem with the input files.

Could you please check whether there is a known issue with the Alpha/Beta diversity modules at the moment?

Dear Chiara,

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Thanks,

Guangyan