What does the Seq2Fun algorithm do?

Seq2Fun is an ultrafast, all-in-one functional profiling tool for raw RNA-seq reads quantification for organisms without reference genomes. Seq2Fun works by searching cleaned and translated reads against a high-resolution protein ortholog database that combines sequence information from ~600 species.

Seq2Fun generates a counts table (Seq2Fun Ortholog IDs X Samples) that can be analyzed with conventional RNA-seq analysis pipelines in R, or through our ExpressAnalyst tool.