OmicsAnalyst accepts two or more feature abundance tables generated from common omics technologies such as transcriptomics, proteomics, metabolomics, microbiome, and miRNA data.
Features must be in rows and samples in columns (example below). Files must either be in .txt, .csv, or their compressed format (.zip).
Example Abundance File (containing metadata)
#NAME sample10 sample105 sample11 sample113
#CLASS:Condition Classical Classical Classical Classical
FSTL1 0.04085 1.09922 -0.45374 0.03402
MMP2 1.76569 -0.50303 0.41764 1.25827
BBOX1 0.9542 1.21379 0.95196 0.60273
GCSH 0.59383 0.66385 -0.00448 0.47665
EDN1 0.8455 -0.26195 -0.34848 0.08309
CXCR4 0.1691 0.04824 0.47361 -0.55183
SALL1 0.45267 0.97534 0.35652 1.32005
MMP7 -1.47079 -1.29109 -1.40518 -1.08673
......
Example Abundance File (metadata in a separate file below)
#NAME sample10 sample105 sample11 sample113
FSTL1 0.04085 1.09922 -0.45374 0.03402
MMP2 1.76569 -0.50303 0.41764 1.25827
BBOX1 0.9542 1.21379 0.95196 0.60273
GCSH 0.59383 0.66385 -0.00448 0.47665
EDN1 0.8455 -0.26195 -0.34848 0.08309
CXCR4 0.1691 0.04824 0.47361 -0.55183
SALL1 0.45267 0.97534 0.35652 1.32005
MMP7 -1.47079 -1.29109 -1.40518 -1.08673
......
A metadata file (upload together with the above feature abundance file)
#NAME Condition Gender
sample10 Classical M
sample105 Classical M
sample11 Classical F
sample113 Classical M
......
Notes about formatting your data files:
- Sample and feature names must be unique and consist of a combination of common English letters, underscores and numbers for naming purpose. Latin/Greek letters are not supported.
- Sample names must be consistent across all files (i.e. omics abundance tables and metadata file).
- Data values should contain only numeric values. Empty cells or cells with NA values are considered missing values.
- If you include metadata in the feature abundance file, metadata or group label should directly below sample names indicated by “#CLASS”. You can indicated multiple factors. For instance, the above TCGA example data sets contains two factors indicated by “#CLASS:Condition”, “#CLASS:Hours”, respectively.