Using picrust if data if you used curated greengens v13.5 and curated microseq 16s reference

I am trying to do functional predictions using microbiome analyst. I did bioinformatics analysis using ion reporter software and the unaligned binary data generated by Ion S5. Curated Greengenes V13.5 and curated MicroSEQ 16S reference library V2013.1 were used as the reference for the identification of conserved regions. Now I am trying to use microbiome analyst (picrust) to predict functions, I keep getting an empty error. what might be the problem? can I even use Microbiome analyst with data aligned with those curated reference ?