Hello, I’ve been using the microbiome analyst meta-analysis module. I have 3 datasets which I would like to do the batch correction and perform the standard diversity analysis (ie alpha, beta). I have faced 2 issues:
1. Although I can upload the files in the free version, I keep getting the error message on the pro version.
2. Although each individual study can be entered into MA, and I can do the beta diversity (Aitchison(Euclidean) Distance Metric with CLR), the moment I try in the meta-analysis function I get the error in the 2nd photo.
The detailed processes and steps are as follows:
1. The 3 files are annotated to HOMD (so I selected not specified/others)
2. I applied the standard filters (Variance 15%, minimal count 4 and 10% abundance, followed by CLR transformation and applied adjust study batch effect.
3. At the meta-analysis parts, I am unable to do the alpha/beta diversity (error message pops up). I am however, able to visualize the abundance in the bar charts.
a. When I click the biomarker discovery part it just doesn’t move to the next page.
Could I seek help to trouble I’ve attached the count and meta files here.
COUNT study 1.csv (95.4 KB)
COUNT Study 2.csv (40.8 KB)
COUNT Study 3.csv (195.0 KB)
META Study 1.csv (1.2 KB)
META Study 2.csv (628 Bytes)
Meta Study 3.csv (3.3 KB)
shoot these issues?
