Troubleshooting issues with upload for Statistical Meta-Analysis

MicrobiomeAnalyst
1

Greetings
Within the Statistical Meta-Analysis Package, when I try to upload my data, I receive the following: “This page indicates an unknown error that is unique to your data and parameters. We suggest the following steps…”

It is asking me if everything is formatted correctly. I don’t see an error in the uploaded files. Could you please take a look and let me know what might be wrong?

Data uploaded:
metadata.txt (5.1 KB)
MS2.txt (245.3 KB)
TAXONOMY.txt (45.4 KB)

With MS2 file for OSU/ASV table, I didn’t select taxonomy included, sequences included, or normalized data.
For taxonomy, I’ve tried SILVA taxonomy and Not Specific/Other.

The data were generated using DADA2 for R.

Thanks for any help.

2

Hello,

The meta-analysis is dedicated to the analysis of multiple datasets. If you have only one dataset as attached here, we recommend the MDP module to do the analysis.

Hope this helps!

3

Greetings, thank you for your reply. When I try MDP with the same uploads, I get:
“Error! Possible causes of error (last one being the most relevant): Failed to read in the OTU abundance data! Please make sure the data is in the right format and do not have empty cells or NA.”

I think that the files are in the correct format. Could you please let me know if I’m missing something?
metadata.txt (5.1 KB)
MS2.txt (245.3 KB)
TAXONOMY.txt (45.4 KB)

Thanks!

4

Hello, this is because you have two blank rows at the bottom of your OTU table like this:

image
image1405×165 3.46 KB

Please delete these two blank rows and it should work. There should not be missing value or blank value in the OTU table.

Hope this helps!

1 Like
5

Thank you so much! Not sure how the extra rows got there, but removing those fixed it. I’m so grateful!