I have successfully run tax4fun (with help of folks in this forum) on some data sets. However, I’ve run into a novel snag with this one. Here are the files:
Q_ASVs.txt (349.9 KB)
Q_Metadata.txt (11.9 KB)
Q_taxonomy.txt (79.0 KB)
I am using tax4fun, QIIME against SILVA database.
My student obtained the classifier file from Data resources — QIIME 2 2023.5.1 documentation
I get this error:
"Samples Q110 . . . (includes list of samples) . . . NA, NA, NA were removed during the processing due to the abundance of all the matched taxa are 0 in these samples.
I looked at the samples in this list, and I don’t see problems with the counts. Is this an issue related to the Silva database?
Here is the output file:
functionalprof_tax4fun-2.csv (255.5 KB)
Odd, in that I only get data for the samples that the warning said were removed.