Hey,
I’m trying to analyse some fastq files using the Raw Data Processing. The protocol is filtering over 95% of my reads, its going from 50000 reads to 1000. However, when a friend runs the same fastq file through the dada 2 pipeline on R over 20000 reads remain after filtering.
I ran the same files through the free version and over 80% of the reads pass the filter however!
Is anyone else having a similar issue with the new version of uBA Pro?
Thanks in advance for your help with this matter.
Ciao
Cris
Hello,
Thanks for bringing up this issue. Could you please share several sequence data with us for testing purposes?
Since our example data works well (same in public and pro version), we might need the data to reproduce the issue. If you prefer not to upload it here, you can send a small subset of your data via email.
Best,
Yao
sure, but where do I send the meta data and fastq files?
Cris
Also, I noted that when changing the parameters for the filtering, they are not updated, as indicated by the R script file.
Cris
Hello, I am still waiting to hear back from you to understand where I can send the fastq and metadata files. The contact page just says to contact you via the forum…
Ciao
Cris
Hello,
I missed your previous message. Sorry about it. You can send to my email yao.lu5@mail.mcgill.ca.
Best,
Yao
This topic was automatically closed after 7 days. New replies are no longer allowed.