Problem with microbial metabolomics analysis

Hi, I analyzed the microbiome data using HUMAnN 3.0, and MetaPhlAn 4.0. I used their results as an input for the microbial metabolomics analysis. The problem I had was it requested groups in the meta data , but my samples was for one group of MS patients. Can I proceed with microbial metabolomics with my type of dataset?

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