Metabolome-wide statistical comparison

Hi all,

The sPLS-DA provides a nice visualization of the differences in metabolomes between samples. Is there a statistical methods to support strong clustering within metaboanalyst? I am thinking of something along the lines of an ANOSIM or PERMANOVA.

Thank you,
Tyler


Tyler J. Carrier, Ph.D.
Department of Biological Sciences
University of North Carolina at Charlotte
Charlotte, NC 28223
e-mail: tcarrie1@charlotte.edu

It is certainly possible to apply the clustering assessments to PCA (similar to PCoA employed in ecological data analysis).

Not sure if it is very meaningful or useful to apply this to sPLS-DA. The common practice is cross validation and permutation tests for supervised methods.

Hi Jeff,

I can also use PCA; that is no problem. How would one perform an ANOSIM or PERMANOVA–or something similar–using the online version of MetaboAnalyst? I did not see this option. I say ANOVAs for each metabolite, but not a ‘community-wide’ analysis.

-Tyler

No, this is not available in MetaboAnalyst. Maybe you can try our MicrobiomeAnalyst which supports this practice from the very beginning.

Hi Jeff,

But the data are not related to the microbiome, so how would that work?

-Tyler