Issue with PICRUSt Functional Prediction

MicrobiomeAnalyst
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1

Hello All,

I want to perform a functional prediction with my 16S sequence data using PICRUSt. I’ve created a BIOM file with taxonomy in QIIME, along with a metadata file, to upload to MicrobiomeAnalyst (see link). I am able to filter and normalize my data, and all of the analyses except the functional prediction seem to be working fine. When I try to run the PICRUSt functional prediction, I receive this message:

Error

Possible causes of error (last one being the most relevant):
null
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I have checked that I am using a compatible version of the GreenGenes database (13.5). I have linked a folder containing the BIOM file and metadata file I am uploading, along with the taxonomy file used to make the BIOM file. Does anyone have insight into what my issue may be?

Omics_Shared_files

Thanks in advance,

Jace

2

Unfortunately, the files are empty (or could be permission issues). MicrobiomeAnalyst currently supports PICRUSt v1 . I would suggest to use Tax4Fun2 directly on your ASV data.

3

Hi Jeff,

Sorry about that. You should be able to download (and hopefully view) my files using this link: Omics_Shared_files

Thanks,

Jace