Issue with Dose-response analysis

[mhannah1314]

Hi!
I keep getting an error message with I am trying to generate tPods for my data. These are the same files I used successfully on fastBMD, but for some reason they aren’t working on ExpressAnalyst.

Data upload and normalization works fine:

And I am able to get DEGs

But I keep getting an error for tPoD generation

The data file with metadata included is attached below:
fast_bmd_pfos_2 copy.txt (3.1 MB)

I am not sure what to do as it was working fine previously.

[Guangyan]

I downloaded your data and chose the exact same parameters and I was able to generate tPODs. Could you try again?

[mhannah1314]

I still get the error with curve fitting.

[AJ_Alcaraz]

Seems like the issue is with DESeq2. Try using TMM then edgeR. It should work.

[Guangyan]

Hello AJ,

The issue with DESeq2 is now fixed. Can you try again?

Guangyan