I can’t upload my data, it says I have problems on my metadata

MicrobiomeAnalyst
1

Hi, I didn’t reply on time and the later topic was blocked, I know that the problem is my metadata but I just don’t understand how can I change it, I have 4 samples only and they don’t share any characteristics so all data will be different between them. my question is how can I do my metadata to do the analyses.

My actual metadata file:

|#NAME|SampleType|
|BC1|biofilm|
|BC2|water|
|BC3|water|
|BC4|soil|

the error:

  • ErrorMetadata Table: make sure there is at least one column contains experimental design for group comparisons (i.e., the primary metadata), with each group contains at least 3 replicate. No unique values are allowed in the primary metadata column.
2

MicrobiomeAnalyst is mainly designed for statistical analysis - a minimum data should have two groups with 3 replicates each. The error message literally tells you that your data containing 4 samples is NOT SUPPORTED.

3

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