Hi, I didn’t reply on time and the later topic was blocked, I know that the problem is my metadata but I just don’t understand how can I change it, I have 4 samples only and they don’t share any characteristics so all data will be different between them. my question is how can I do my metadata to do the analyses.
My actual metadata file:
|#NAME|SampleType|
|BC1|biofilm|
|BC2|water|
|BC3|water|
|BC4|soil|
the error:
- ErrorMetadata Table: make sure there is at least one column contains experimental design for group comparisons (i.e., the primary metadata), with each group contains at least 3 replicate. No unique values are allowed in the primary metadata column.