How is the raw RNA-seq data processed?

After data uploading, ExpressAnalyst performs three main steps to process raw RNA-seq data.

  1. Quality checking
  2. Feature (transcripts, ortholog groups) quantification for each sample
  3. Summarization across samples to generate a count table

The count table can be uploaded to ExpressAnalyst for statistical and functional analysis. The Figure below shows the overall workflow (the steps 3.1 - 3.2 - 3.3 correspond to the 3 steps)