After data uploading, ExpressAnalyst performs three main steps to process raw RNA-seq data.
- Quality checking
- Feature (transcripts, ortholog groups) quantification for each sample
- Summarization across samples to generate a count table
The count table can be uploaded to ExpressAnalyst for statistical and functional analysis. The Figure below shows the overall workflow (the steps 3.1 - 3.2 - 3.3 correspond to the 3 steps)