I tested it and found some small problems.
When the Taxonomy level is Family, similar errors will still occur.
and ,In some cases, there is no text display on the Heat Tree
Have you checked the parameters, such as the Wilcoxon p-value cutoff? The new error might be due to no difference detected given the cutoff you set. I increase it to 1(just for explain the issue), then I can get the figure below:
…nor does SparCC run. Do you have an idea where could be the mistake in my data sets? Up to now (step 14), everything worked like stated in Chong et al. (2020). Thank you!