Hello,
I uploaded my fastq.gz (pair-end sequencing) and a metatable, but stuck on step 2, it always shows **ERROR! No reads passed the filter.**Please revisit your filtering parameters", my quality scores are over 30 and 40, so i have changed the trunc length to 0, but it still didn't work. I tried to set all parameters to 0, and it still doesn't work. I tried to visualize this data set using microbiomeAnalyst2.0 and it worked several days ago, but starting from yesterday, it always shows this error.
Can anyone offer some insights? it is the same fastq.gz and metatable that I used. Please let me now how to solve this error! Thank you!