Error in Normalisation

Hello,

I am trying to analyse RNA count data.

I’m receiving the Error: failed to perform normalization when I check any of the transformation options including “none”. I can’t see anything wrong with the data and all downstream analyses work…

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https://www.dropbox.com/scl/fo/aoqgb4381n1qh3ivsynix/AIKciYG8AZX1gOSn27E9vck?rlkey=uy775ydg8l4jmbdhz8bwyw47b&st=8ytutrmp&dl=0

Can you please see the data and metadata in this dropbox?

This is unannotated and no model organism, so the option is turned off. Have tried with abundance and variance at 0, or any random number, and normalization “none” or any option and always an error…