Issues with DEG analysis

[EmilyB]

Hi,

Please see the data below for you to help me with my issue:

1. Express analyst → DEG analysis

2. Data uploaded
   S2fid_abundance_table_all_samples_submit_2_expressanalyst_trial 53.txt (2.4 MB)

3. Document all steps leading to the issue.
   Step 1: Input: A gene expression table
   Step 2: Upload data (see screen shot)
   

   

Step 3: Data normalization (see screen shot)

Step 4: DEG analysis (see screen shot with error message)

How come, I can no longer use EdgeR (with normalized data) for DEG analysis? Also, when I use the suggested Limma for normalized data I get an error message…