Data Upload Issue in MicrobiomeAnalyst marker data profiling

MicrobiomeAnalyst
1

I’m encountering an issue when trying to upload my data into MicrobiomeAnalyst. I receive the following error message: Error: Please check the file format of uploaded files! The format should be one of txt, csv, biom, shared, taxonomy, tre, nwk, zip depending on the requirements of different modules!

:one: Tool and Module: MicrobiomeAnalyst - Marker Data Profiling
:two: Data Provided: I have three input files –

  1. ASV Table: txt file with #NAME in the first row and ASVs as rows
  2. Taxonomy Mapping File: txt file with taxonomy as the first column header
  3. Metadata File: txt file with #NAME as the first column header

(Attaching small sample formats for all three for reference.)

:three: Steps Leading to the Issue

  1. Uploaded ASV table, taxonomy mapping file, and metadata file and selected SILVA taxonomy as Taxonomy labels
  2. Clicked “Submit”
  3. Received the file format error listed above.

**Additional Notes: I formatted the ASV table and taxonomy mapping file following the guidelines (tab-separated .txt format).

Could you please confirm if there’s a formatting issue with my files, or if they need to be combined differently before upload?
metadata.CSV (1.9 KB)
taxonomic_mapping_file.CSV (317.3 KB)
ASV_table_sample.csv (5.7 KB)

Thank you for your help!

2

If the full dataset is needed to investigate this issue, please let me know. I can provide a de-identified version via email for your review.

3

Hello Hannah,

I have checked your data and found a small issue: the file extension should be .csv instead of .CSV. Please correct it and try again. Hope this helps and let us know if you have further questions.

Best,
Yao

4

Great! Now it uploads without issue. Thanks for your response!